When studying the evolution of books from ancient times until the present, it is important to be aware
of the simultaneous development of not only the text and its meaning, but also the different
techniques and materials used through the ages. From approximately the 3rd century AC until the end
of the Medieval Ages, when printing on paper was first introduced, manuscripts, scrolls, and book
covers were manufactured using parchment, a material derived from animal skin, primarily sheep, calf,
or goat. The main constituent of parchment is collagen, a protein with a triple-helix structure.
Depending on the environmental conditions under which the medieval manuscripts are presented or
stored (e.g., temperature, relative humidity, acidifying gases in the ambient atmosphere, and light),
and depending on the composition of inks and pigments applied in the text and for the colorful
miniatures (also called illuminations), collagen can undergo several degradation phenomena. These
processes weaken the parchment structure, rendering it fragile and brittle. Moreover, they can induce
a dark brown discoloration of the pigmented area, followed by mechanical damage (cracks), and,
finally, loss of material (formation of holes). To study these phenomena, mock-ups of parchment, inks,
and the combination of the two, will be prepared solely by following medieval recipes and procedures,
using traditional materials (e.g. natural oak galls for the inks), to simulate original objects as closely as
possible. In this way, it will be possible to reproduce medieval manuscripts, undergoing laboratory-
controlled accelerated aging to simulate the effect of time on the objects and therefore their
deterioration pathways. This will allow not only the identification of the deterioration factors but also
to reconstruct the causes and chemical processes occurring during the aging. The combination of non-
invasive analyses performed by compound-specific analytical methods such as Fourier Transform
Infrared (FTIR) and Raman spectroscopy, as well as micro-invasive high sensitivity techniques such as
Electron Paramagnetic Resonance (EPR) spectroscopy, are powerful tools for the investigation of
deterioration processes occurring in proteinaceous materials. This strategy will allow not only to gain
information about the deterioration phenomena found on the surface of the material but will also
enhance the understanding of the chemical (deterioration) processes occurring deeper within the
material. Following this approach, the complementarity of the information obtained will help to detect
and identify the most relevant aging phenomena. It will therefore contribute to a more precise
evaluation of the deterioration status of an object, to ensure a more effective and precise conservation
strategy.